Data/hora: 13/05/2024 04:28:19

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	29/07/2015	
Data da última atualização: 	18/05/2023	
Autoria: 	RAMOS-GONZALEZ, P. L.; SARUBBI-ORUE, H.; GONZALES-SEGNANA, L.; CHABI-JESUS, C.; ASTUA, J. de F.; KITAJIMA, E. W.	
Afiliação: 	PEDRO LUIS RAMOS-GONZALEZ, IAC; HUMBERTO SARUBBI-ORUE, Universidad Nacional de Asuncion; LUIS GONZALES-SEGNANA, Universidad Nacional de Asuncion; CAMILA CHABI-JESUS, IAC; JULIANA DE FREITAS ASTUA, CNPMF; ELIOT WATANABE KITAJIMA, ESALQ.	
Título: 	Orchid Fleck Virus Infecting Orchids in Paraguay: First Report and Use of Degenerate Primers for its Detection.	
Ano de publicação: 	2015	
Fonte/Imprenta: 	Journal of Phytopathology, 2015.	
Páginas: 	6p.	
Série: 	1439-0434	
Idioma: 	Inglês	
Conteúdo: 	The presence of Orchid fleck virus (OFV) in Paraguay was confirmed in orchid plants collected during a survey carried out in 2013. Leaves displayed ringspot and fleck symptoms, and in infected tissues, non-enveloped, short, rodlike viral particles were observed. Partial OFV N and L genes were amplified using specific and degenerate primers, respectively; the nucleotide sequences demonstrated high identities (98%) with other OFV isolates. Degenerate primers for the L gene were designed considering conserved regions within all of the available OFV sequences and those from the closely related isolates citrus leprosis virus nuclear type (CiLV-N) and citrus necrotic spot virus. Degenerate primers were also successfully used for the detection of CiLV-N from infected citrus samples.	
Palavras-Chave: 	Citrus leprosis virus.	
Thesagro: 	Doença de planta; Orchidaceae; Orquídea.	
Categoria do assunto: 	O Insetos e Entomologia	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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